PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
Leaves N-glycan core oligosaccharides intact and suitable for further analysis
Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
≥ 95% purity, as determined by SDS-PAGE and intact ESI-MS
Stored in 50% glycerol
Optimal activity and stability for up to 24 months
Can be used under native or denaturing conditions